Variable Binding of Thioflavin T to Amyloid Fibrils
نویسندگان
چکیده
منابع مشابه
Thioflavin T Binding to Amyloid Fibrils
Treatment of Alzheimer’s disease (AD) is hampered by the fact that the disease progression cannot be tracked in vivo at this time. Understanding the properties of Aβ amyloid fibrils associated with AD is imperative to finding a way to track the progression of the disease. Recently, fluorescent markers, derived from thioflavin T have been developed as markers of AD. This project examines the int...
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1. Experimental details All reagents were purchased from Aldrich, the commercial ThT(Cl) was recrystallized in acetonitrile. The slow addition of an aqueous saturated solution of KI (5 ml) to an aqueous solution (20 ml) of (ThT)Cl (0.600 g, 1.88 mmol) resulted in the formation of a yellow solid that was filtered off, washed with cold water (10 ml) and dried in vacuo. MS(+ESI-MS) showed the pres...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2019
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2018.11.1079